Impact of treated effluent discharges on fish communities: Evaluating the effects of pollution on fish distribution, abundance and environmental integrity.

Domestic effluent discharges change water quality and habitat conditions in urban watersheds, though less known about how these impact fish communities. This work assessed the impact of chronic wastewater pollution on biotic and abiotic factors in six urban streams in Patagonia. Stream hydrological features, water quality conditions and fish communities were analyzed during a one-year period. The oxygen saturation and water velocity showed significant differences between up- and downstream locations of wastewater treatment plants (WWTPs). Chemical parameters revealed an eutrophication process downstream of the WWTP input, with increased biological oxygen demanding (BOD), nitrogen, ammonium, soluble reactive phosphorus, and chlorophyll a concentrations, indicating nutrient enrichment that can lead to a potential for algal growth. The study highlighted significant differences in fish abundance, density, and biomass between reaches upstream (Control) and downstream (Impacted) of the WWTP discharges, suggesting a detrimental impact on fish communities. While juveniles, females and males of the native Catfish (Hatcheria macraei) preferred pristine zones, juveniles and males of the native Perch (Percichthys spp.) displayed preferences for areas with higher nutrient levels. Exotic species like Rainbow Trout (Oncorhynchus mykiss) (juveniles, females and males) preferred low-nutrient, high-quality habitats, while juveniles, females and males of Brown Trout (Salmo trutta) were found near the WWTP facilities. Although some previous studies have suggested that nutrient enrichment might benefit fish, our findings highlight the negative impacts on fish abundance and distribution due to WWTPs. Fish species appear to demonstrate certain degrees of tolerance to pollution, with larger individuals displaying greater tolerance. Although the pollution levels may did not result in an irreversible collapse of the system, the absence of fish in the stream with the highest pollution level would indicate an ongoing environmental deterioration. Anthropogenic activities, especially municipal effluent discharge, exacerbate environmental degradation and demand specific management actions to maintain ecosystem integrity.

Flow regulation by dams impacts more than land use on water quality and benthic communities in high-gradient streams in a semi-arid region.

In semi-arid regions, water policy has strongly promoted the construction of water reservoirs with little or no consideration for their ecological consequences. In order to quantify the effect induced by flow discontinuity on environmental conditions, water quality, and invertebrate communities at high-gradient streams, we investigated unregulated and regulated reaches at 13 watercourses, located in the Dry Chaco Ecoregion (South America). Dams differed in the dominant land uses (rangeland, agriculture, and urban) of the related catchment area. We assessed on-site hydro-geomorphic features, water quality and bacteriological parameters, habitat condition, chlorophyll a, macrophytes cover, and macroinvertebrate communities. Significant increases in mineral parameters and organic contamination indicators were detected at regulated reaches, such as: conductivity, total solids, turbidity, color, and phosphates. Dams negatively affected habitat condition, and macrophyte cover increased at regulated sites. Macroinvertebrates showed a diminution in most of the metrics analyzed, with a decrease of sensitive groups and an increase in the more tolerant ones. Redundancy Analysis revealed that SWQI (physicochemical based index) and the proportion of coarse gravel were stronger predictors on metrics arrangement. Variance partitioning analyses proved that regulation effects prevailed over land use in explaining metrics variation. Invertebrate community was positively related to better ecological conditions, which suggests that restitution of ecological integrity at regulated reaches should include habitat restoration. These results are relevant for the management of regulated water resources in arid and semi-arid regions in a context of climate change.

Multixenobiotic defence mechanism in native and exotic freshwater snails as a biomarker for land uses-changes.

Human activities such as agriculture and urbanization generate a large number of substances like personal care products, pharmaceutical compounds, and pesticides, which often reach aquatic environments and represent a threat to biodiversity. Many organisms have developed different evolutionary strategies to remove pervasive substances from their bodies, allowing them to persist even in polluted environments, and one of these is the multixenobiotic resistance (MXR) mechanism associated with the expression of membrane proteins like P-glycoprotein (P-gp). Numerous chemical compounds with diverse functions and structures can modulate this mechanism, which can be employed as a pollution biomarker. We examined the MXR activity in two species of snails that inhabit Patagonian freshwaters. Functional assay measurements of MXR were conducted on the native Chilina dombeiana and the exotic Physella acuta in stream reaches affected by anthropogenic impacts. Results indicated that at agricultural sites, C. dombeiana snails had a more active MXR system than organisms sampled at reference and moderately disturbed urban sites, whereas P. acuta snails from agricultural and highly disturbed urban sites showed better detoxifying activity than organisms from reference sites. Only in exotic snails, part of this activity was due to the action of P-gp. The most important environmental variables explaining MXR activity were ammonium, nitrate and nitrite, phosphates, and electrical conductivity. These results show the promise of measuring MXR activity in native and exotic snails, as a biomarker in the environmental monitoring of Patagonian freshwaters.

Variability in Nutrient Dissipation in a Wastewater Treatment Plant in Patagonia: A Two-Year Overview.

Constructed wetlands are environmental solutions that mitigate the impacts of urban effluents. It is unclear how the performance of these wastewater treatment plants (WWTP) is affected by climatic conditions. The dissipation of nutrients, suspended solids, and changes in dissolved oxygen were investigated on a monthly basis over two years (2018/2019) at six sampling points across a WWTP located in Esquel, Patagonia. It was predicted that climatic variables (rain pattern and air temperature) would affect the functioning and efficiency of the WWTP (i.e., via nutrient load mitigation and sediment retention). Rainfall and temporal patterns differed markedly between and throughout the two years, leading to a clear seasonality in the transformation of pollutants. Nitrate loads were significantly higher in 2018 than in 2019 suggesting some degree of operational failure, whereas ammonia levels in treated effluents were extremely high during both years, with marked peaks occurring during autumn 2018 and summer 2019. The WWTP was moderately successful (~36%) in reducing TSS contents during 2018 but was inefficient in 2019. Ammonia levels in receiving waters underwent dilution due to rains rather than due to adequate WWTP nutrient retention. In terms of nutrients, effluent values exceeded those established by governmental regulation during most months, but worsened during summer coinciding with low flows. This lack of predictability for the values of the treated effluent strongly jeopardizes the ecological integrity and biodiversity of the receiving stream.

Impact of Chaitén Volcano ashfall on native and exotic fish recovery, recolonization, and abundance.

The effects of volcanic disturbance on aquatic communities and their recovery are poorly studied. To fill this gap, we explored the effects on fish communities in rivers in Argentina of the 2008 eruption of Chaitén Volcano in southern Chile (42.8° lat. S). The eruption produced volcanic plumes of ash that persisted in the atmosphere for several months. Borne on westerly winds, deposits of tephra crossed the Andes Mountains, reaching the Atlantic coast (Argentina). We compared the pre- and post-eruption abundances of a native catfish Hatcheria macraei, and two introduced trout from rivers covered by the volcanic plumes (Argentina) using Before-After-Control-Impact analysis to explore fish recovery. Total suspended solids from volcanic ashfall, macroinvertebrate abundance and richness, and species ecological attributes influenced the spatial arrangement of fish in rivers. Twenty-one months after the eruption, Rainbow Trout, Oncorhynchus mykiss, had not returned to pre-eruption abundances in the sampled rivers, and only four rivers had regained pre-eruption species composition, suggesting that disturbance is still ongoing. The abundance of introduced fishes was strongly, negatively correlated with TSS, suggesting that ashfall affected these fish probably by clogging and abrasion of the gills. Fish recolonized previously occupied habitats 4 days to 9 months after the disturbance. Hatcheria macraei was the slowest to recolonize, whereas O. mykiss were the pioneer fish in 4 rivers following the eruption and recolonized all 5 rivers where they were present prior to the eruption. In one river, the catfish and the Brown Trout, Salmo trutta, were still absent 21 months post-eruption, potentially owing to the lack of riparian cover that would have deflected the entry of ash. Rainbow Trout suffered significant declines in abundance, whereas Brown Trout and catfish generally did not, owing to their ecological attributes. Total fish abundance was negatively correlated with ash thickness, but positively related to prey availability.

Ecological drivers of alpha and beta diversity of freshwater invertebrates in arid and semiarid Patagonia (Argentina).

Freshwater biodiversity is showing a global decline in relation to environmental change and there is an urgent need to quantify different facets of biodiversity to assess the conservation value of freshwater ecosystems. Identifying the ecological drivers of arid and semiarid Patagonian waterbodies is a critical requirement for planning action to conserve these still poorly understood habitats. We tested the responses of alpha and beta diversity of invertebrates (presence-absence, abundance, and biomass) to local, landscape, and climatic features, in 45 Patagonian freshwater bodies classified in three groups: isolated ponds, connected ponds, and lotic systems. We first compared the invertebrate community compositions of the different waterbodies. Secondly, using a co-inertia analysis we assessed the relative roles of local, landscape and climatic factors in driving variation in assemblage composition. Finally, we compared different facets of beta diversity and modelled their responses to environmental variables (predictors), by generalized dissimilarity modelling (GDM). Unexpectedly, lotic systems with a total of 109 taxa, were richer than lentic ones, but isolated ponds contributed most to beta diversity and supported more unique and proportionally more endemic species. Alpha and beta diversity were mainly driven by local features and secondly by climatic descriptors. However, these patterns shifted according to different community composition measures. Collectively, our results suggest that isolated ponds have a higher biodiversity value and would be more vulnerable than connected ponds and lotic systems to environmental changes. However, all waterbody types contributed to gamma biodiversity, reinforcing the importance of conserving a diversity of freshwater ecosystems in any landscape and particularly in arid and semiarid Patagonia. Our findings are a first step to resolving and quantifying the amount of changes occurring in Patagonian waterbodies, and are a prerequisite for predicting changes in species distributions in the face of global change.

Biotic diversity of benthic macroinvertebrates at contrasting glacier-fed systems in Patagonia Mountains: The role of environmental heterogeneity facing global warming.

Patagonia is by far the largest glacierized area in South America. However, little is known about ecology, functioning and biodiversity of glacier-fed streams facing global warming. We investigated changes in environmental features and macroinvertebrate communities along a longitudinal gradient of glacier influence of two Patagonian systems that differ in glacier cover magnitude and the spatial sequence of lotic and lentic phases. Both glaciers, Torrecillas (~5.5km2, Torrecillas system) and Cónico (~0.44km2, Baggilt system), are retreating. Longitudinal distribution of benthic invertebrates partially fitted to predictions for glacierized temperate systems, with Diamesinae spp. dominating at closest sites to the Cónico, and Orthocladiinae increasing downstream, but patterns were unclear at Torrecillas. Generalized Linear Model identified chlorophyll a and conductivity as having significant effect on richness and density respectively at Torrecillas; detritus biomass and gravel influenced species richness, and boulder percentage and water temperature affected density, at Baggilt. Canonical Correspondence Analyses integrating benthic biota and environmental variables revealed that a higher environmental heterogeneity at Baggilt, related with spatial dimension (unshaded/shaded reaches, wetland reaches), local resources (detritus, bryophytes) and temperature, probably explained the unexpected high richness in benthic assemblages (67 taxa). Environmental conditions imposed by the lake outlet (proglacial) at Torrecillas resulted in a less diverse community (31 taxa). Finally our results suggest that these isolated, small glacier-fed streams typical of the Patagonian landscape appear highly vulnerable to global warming. Endemic elements could disappear at upper segments being replaced by other species common at rhithral environments, which might increase local diversity (alfa diversity) but decrease regional diversity (gamma diversity). From an ecosystem perspective stream functioning can result altered. Glacier retreating or disappearing threatens major ecosystem services for Patagonian inhabitants such as water supply, hydrological regulation, recreation and tourism.

Environmental Quality and Aquatic Invertebrate Metrics Relationships at Patagonian Wetlands Subjected to Livestock Grazing Pressures.

Livestock grazing can compromise the biotic integrity and health of wetlands, especially in remotes areas like Patagonia, which provide habitat for several endemic terrestrial and aquatic species. Understanding the effects of these land use practices on invertebrate communities can help prevent the deterioration of wetlands and provide insights for restoration. In this contribution, we assessed the responses of 36 metrics based on the structural and functional attributes of invertebrates (130 taxa) at 30 Patagonian wetlands that were subject to different levels of livestock grazing intensity. These levels were categorized as low, medium and high based on eight features (livestock stock densities plus seven wetland measurements). Significant changes in environmental features were detected across the gradient of wetlands, mainly related to pH, conductivity, and nutrient values. Regardless of rainfall gradient, symptoms of eutrophication were remarkable at some highly disturbed sites. Seven invertebrate metrics consistently and accurately responded to livestock grazing on wetlands. All of them were negatively related to increased levels of grazing disturbance, with the number of insect families appearing as the most robust measure. A multivariate approach (RDA) revealed that invertebrate metrics were significantly affected by environmental variables related to water quality: in particular, pH, conductivity, dissolved oxygen, nutrient concentrations, and the richness and coverage of aquatic plants. Our results suggest that the seven aforementioned metrics could be used to assess ecological quality in the arid and semi-arid wetlands of Patagonia, helping to ensure the creation of protected areas and their associated ecological services.

A comparative analysis reveals weak relationships between ecological factors and beta diversity of stream insect metacommunities at two spatial levels.

The hypotheses that beta diversity should increase with decreasing latitude and increase with spatial extent of a region have rarely been tested based on a comparative analysis of multiple datasets, and no such study has focused on stream insects. We first assessed how well variability in beta diversity of stream insect metacommunities is predicted by insect group, latitude, spatial extent, altitudinal range, and dataset properties across multiple drainage basins throughout the world. Second, we assessed the relative roles of environmental and spatial factors in driving variation in assemblage composition within each drainage basin. Our analyses were based on a dataset of 95 stream insect metacommunities from 31 drainage basins distributed around the world. We used dissimilarity-based indices to quantify beta diversity for each metacommunity and, subsequently, regressed beta diversity on insect group, latitude, spatial extent, altitudinal range, and dataset properties (e.g., number of sites and percentage of presences). Within each metacommunity, we used a combination of spatial eigenfunction analyses and partial redundancy analysis to partition variation in assemblage structure into environmental, shared, spatial, and unexplained fractions. We found that dataset properties were more important predictors of beta diversity than ecological and geographical factors across multiple drainage basins. In the within-basin analyses, environmental and spatial variables were generally poor predictors of variation in assemblage composition. Our results revealed deviation from general biodiversity patterns because beta diversity did not show the expected decreasing trend with latitude. Our results also call for reconsideration of just how predictable stream assemblages are along ecological gradients, with implications for environmental assessment and conservation decisions. Our findings may also be applicable to other dynamic systems where predictability is low.

Selective foraging by non-native rainbow trout on invertebrates in Patagonian streams in Argentina.

BACKGROUND: It is well known that fish predation alters ecosystem processes by top-down effects. Salmonids are described as aggressive, visually and size-selective predators. Thus, prey selection by the non-native rainbow trout was examined on a seasonal basis at two streams: Nant y Fall (NyF) and Cabeza de Vaca (CVA) at Patagonia, a region where this kind of information is lacking. RESULTS: The benthos density at NyF was higher than that at CVA, and at both streams, riffles supported higher macroinvertebrate densities than pools. The diet of trouts from both streams was dominated by aquatic macroinvertebrates, was diverse, and was varied seasonally. The individuals represented in the stomachcontents were among the largest available at the streams. Diet diversity peaked during spring at NyF and during summer at CVA, whereas at both streams, the niche width peaked during spring. Preyselectivity varied seasonally. The selected preys included both aquatic (Gasteropoda, Crustacea, Plecoptera, Trichoptera, Ephemeroptera, Coleoptera, Diptera, and Odonata) and terrestrial organisms (adult dipterans, Oligochaeta, Araneae, Homoptera, Hymenoptera, Orthoptera, and Hemiptera). Some infaunal invertebrates like oligochaetes and some small Coleoptera and Diptera larvae (mainly Chironomidae) were not selected by trouts. CONCLUSIONS: Despite of the overall dominance of trichopteran species, the composition of the diet of the rainbow trout varied seasonally. This fish positively selected both aquatic and terrestrial organisms. We observed that in both streams, trouts consumed the larger individuals available in those environments.

Does nature and persistence of substrate at a mesohabitat scale matter for Chironomidae assemblages? A study of two perennial mountain streams in Patagonia, Argentina.

Chironomid substrate-specific associations regarding the nature (organic-inorganic) and stability (stable-unstable) of different habitats were investigated at two low order Patagonian streams, during high and low water periods. Nant y Fall and Glyn rivers were visited twice (October 2007 and March 2008) and seven different habitat types were identified. A total of 60 samples were collected using a Surber sampler (0.09 m(-2) and 250 µm) and a set of 23 environmental descriptors including physicochemical parameters and different fractions of particulate organic matter were assessed. 35 Chironomidae taxa were recorded with Orthocladiinae (20), Chironominae (7), and Podonominae (4) being the most well-represented subfamilies. Paratrichocladius sp. 1, Parapsectrocladius sp. 2, Parametriocnemus sp. 1, Pseudochironomus sp., and Rheotanytarsus sp. were the most abundant taxa. According to the relative preference index, at least 14 taxa showed strong affinity for a particular substrate. The structurally complex macrophyte Myriophyllum quitense supported 11 taxa compared with only five taxa found on the less complex Isoetes savatieri. Generally, stable substrates (boulders, cobbles, and rooted plants) supported significantly higher chironomids richness, abundance, and diversity than unstable ones (gravel-sand). Canonical correspondence analysis revealed that detritus (leaves, seeds, and biomass), macrophyte biomass, and secondarily hydraulic variables had high explanatory power on chironomids species composition and structure. This work suggests that more complex substrates showing persistence in the temporal dimension supported a diverse array of chironomids, meaning that the maintenance of natural habitat heterogeneity is essential for the community. Land-use practices having significant effects on ecological stream attributes such as increased turbidity, sediment deposition, and runoff patterns will alter assemblages. Understanding environmental associations of the Chironomidae assemblage at the habitat scale is significant for conservation purposes and for the management of low order streams in Patagonia.

Environmental changes and macroinvertebrate responses in Patagonian streams (Argentina) to ashfall from the Chaitén Volcano (May 2008).

On May 2nd of 2008 the Chaitén Volcano (Chile, 42°50'S and 72°39'W) erupted explosively producing a strong emission of volcanic ash. As a result of this eruption wide areas on the Argentinean side became covered by ashes. In order to investigate the effects of ashfall on environmental features, water quality and macroinvertebrate communities we conducted a study on 10 rivers affected by ash deposition in their hydrographic basins. Sites were visited seasonally (June 2008-March 2010) and results were compared with data obtained from previous research projects. Measures of pH, conductivity, oxygen content, main nutrients, and total suspended solids (TSS) were taken. Macroinvertebrate samples were obtained from riffles and pools. Community attributes were measured and metrics were calculated. A strong and significant increase in TSS values at most sites was recorded and although the peak diminished rapidly during the following months, resuspension and remobilization of ash continue even 20 months after. No significant changes in pH, conductivity and nutrients, comparing with data previous to the ashfall, were detected. Most rivers showed a strong diminution on macroinvertebrate density and richness, being small rivers more severely affected than the big ones. Correspondence analysis based on abundance data allows distinguishing preeruption from posteruption dates at five rivers. Density data and species richness showed low values in March of 2010, indicating that the community was not completely recovered at some sites. At least 25 taxa resulted significantly and negatively affected. Increased mortality could be related to several factors such as habitat deterioration, food quality diminution, interference with breathing mechanisms and with other physiological and morphological characteristics. Specific-taxa responses on the recolonization process were related to dispersal mechanisms and specific strategies.

Assessing land-use effects on water quality, in-stream habitat, riparian ecosystems and biodiversity in Patagonian northwest streams.

Changes in land-use practices have affected the integrity and quality of water resources worldwide. In Patagonia there is a strong concern about the ecological status of surface waters because these changes are rapidly occurring in the region. To test the hypothesis that greater intensity of land-use will have negative effects on water quality, stream habitat and biodiversity we assessed benthic macroinvertebrates, riparian/littoral invertebrates, fish and birds from the riparian corridor and environmental variables of 15 rivers (Patagonia) subjected to a gradient of land-use practices (non-managed native forest, managed native forest, pine plantations, pasture, urbanization). A total of 158 macroinvertebrate taxa, 105 riparian/littoral invertebrate taxa, 5 fish species, 34 bird species, and 15 aquatic plant species, were recorded considering all sites. Urban land-use produced the most significant changes in streams including physical features, conductivity, nutrients, habitat condition, riparian quality and invertebrate metrics. Pasture and managed native forest sites appeared in an intermediate situation. The highest values of fish and bird abundance and diversity were observed at disturbed sites; this might be explained by the opportunistic behavior displayed by these communities which let them take advantage of increased trophic resources in these environments. As expected, non-managed native forest sites showed the highest integrity of ecological conditions and also great biodiversity of benthic communities. Macroinvertebrate metrics that reflected good water quality were positively related to forest land cover and negatively related to urban and pasture land cover. However, by offering stream edge areas, pasture sites still supported rich communities of riparian/littoral invertebrates, increasing overall biodiversity. Macroinvertebrates were good indicators of land-use impact and water quality conditions and resulted useful tools to early alert of disturbances in streams. Fish and birds having a greater ability of dispersion and capacity to move quickly from disturbances would reflect changes at a higher scale.